Description
PART I INTRODUCTION 1 Two-Dimensional Gel Electrophoresis and 2D-DIGE Paula Meleady PART II DIFFERENCE GEL ELECTROPHORESIS 2 Top-down Proteomics and Comparative 2D-DIGE Analysis Kay Ohlendieck 3 DIGE Analysis Software and Protein Identification Approaches Paul Dowling PART III DIGE APPLICATIONS 4 Native DIGE for Quantitative and Functional Analysis of Protein Interactomes Diksha Dani and Norbert Dencher 5 Comparative Two-dimensional Fluorescence Gel Electrophoresis Doreen Ackermann and Simone Knig 6 Identification of Ubiquitination associated Proteins using 2D-DIGE Paul Dowling and Despina Bazou 7 DIGE-based Phosphoproteomic Analysis Taras Stasyk and Lukas Alfons Huber 8 DIGE-based Biomarker Discovery in Blood Cancers Katie Dunphy and Paul Dowling 9 DIGE Saturation Labeling for Scarce Amounts of Protein from Formalin-fixed Paraffin-embedded (FFPE) Tissue Paul Dowling 10 DIGE Analysis of ProteoMiner(TM) Fractionated Serum/Plasma Samples Sandra Murphy and Paul Dowling 11 Comparative 3-sample 2D-DIGE Analysis of Skeletal Muscles Kay Ohlendieck 12 Proteomic identification of saliva proteins as non-invasive diagnostic biomarkers Eleanor O’Sullivan, Paul Dowling, Dieter Swandulla and Kay Ohlendieck 13 Analysis of the saliva proteome using 2D-DIGE Eleanor O’Sullivan and Paul Dowling PART IV COMPARATIVE DIGE TISSUE PROTEOMICS 14 DIGE Analysis of Clinical Specimens Cecilia Gelfi and Daniele Capitanio 15 DIGE Analysis of Animal Tissues Alessio Di Luca, Ruth Hamill, Anne Maria Mullen and Giuliano Elia 16 Rapid 2D-DIGE Proteomic Analysis of Mouse Liver within One Week Shotaro Kamata and Isao Ishii 17 2D-DIGE analysis of liver disease in mice Asha Callebaut, Rita Derua, Lut Overbergh and Veerle Janssens 18 2-Dye Versus 3-Dye DIGE for Comparative Testis Tissue Proteomic Analysis Ashling Holland 19 2-CyDye based 2D-DIGE Analysis of Aged Human Muscle Biopsy Specimens Kay Ohlendieck 20 Identification of Subproteomic Markers for Skeletal Muscle Profiling Paul Dowling, Stephen Gargan, Dieter Swandulla and Kay Ohlendieck 21 DIGE Analysis of Fish Tissues Joanna Nynca, Mariola Dietrich and Andrzej Ciereszko PART V AUXILARY DIGE METHODS 22 Sample preparation and protein determination for 2D-DIGE proteomics Stephen Gargan and Kay Ohlendieck 23 Protein Digestion for 2D-DIGE Analysis Sandra Murphy and Kay Ohlendieck 24 Subcellular Fractionation for DIGE-based Proteomics Sandra Murphy 25 DIGE Analysis of Immunodepleted Plasma Paul Dowling and Kay Ohlendieck 26 Bioinformatic Analysis of the Subproteomic Profile of Cardiomyopathic Tissue Sandra Murphy, Margit Zweyer, Dieter Swandulla and Kay Ohlendieck PART VI DIGE VERIFICATION ANALYSIS 27 Elucidating Cellular Metabolism and Protein Difference Data from DIGE Proteomics Experiments using Enzyme Assays Andrew Dowd 28 Enzyme Assay Methods to Validate DIGE Proteomics Data Andrew Dowd 29 Immunoblot Analysis of DIGE-based Proteomics Martin Landsberger and Heinrich Brinkmeier 30 Verification of Protein Changes determined by 2D-DIGE based Proteomics using Immunofluorescence Microscopy Margit Zweyer, Kay Ohlendieck and Dieter Swandulla 31 Histological and Histochemical Microscopy used to verify 2D-DIGE Pathoproteomics Margit Zweyer, Kay Ohlendieck and Dieter Swandulla
