Description
Macromolecular drugs hold the promise of becoming new therapeutics for several major disorders, including cancer and cardiovascular disease. This incredible potential is explored in Macromolecular Drug Delivery, a volume which gives a wide-ranging overview of contemporary methods used in the field, and which addresses the limitations presented by a lack of safe and efficient drug delivery strategies. Chapters offer information on both in vitro and in vivo methods of macromolecular delivery, thus appealing to a broad scientific audience. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Macromolecular Drug Delivery offers a platform for interdisciplinary collaboration, which should provide opportunities for new discoveries at the interface between disciplines. Ultimately, this cooperation will lead to the use of macromolecular drugs as novel diagnostic tools and, even more importantly, as a means to revolutionize the way we view and treat diseases. Macromolecular Drug Delivery Dr. Mattias Belting, ed. Table of Contents Preface Contributors Developments in Macromolecular Drug Delivery; Review Chapter Mattias Belting and Anders Wittrup Synthetic vs. Natural/Biodegradable Polymers for Delivery of shRNA Based Cancer Therapies; Review chapter John S. Vorhies and John J. Nemunaitis siRNA and DNA Transfer to Cultured Cells Bagavathi Gopalakrishnan and Jon Wolff Poly(-amino esters): Procedures for Synthesis and Gene Delivery Jordan J. Green, Gregory T. Zugates, Robert Langer, and Daniel G. Anderson Systemic Delivery and Preclinical Evaluation of Nanoparticles Containing Antisense Oligonucleotides and siRNAs Chuanbo Zhang, Joseph T. Newsome, Rajshree Mewani, Jin Pei, Prafulla C. Gokhale, and Usha N. Kasid Peptide-based Delivery of Steric-block PNA Oligonucleotides Sad Abes, Gabriela D. Ivanova, Rachida Abes, Andrey A. Arzumanov, Donna Williams, David Owen, Bernard Lebleu and Michael J. Gait Characterizing Peptide Mediated DNA Internalization in Human Cancer Cells Anders Wittrup and Mattias Belting Selection and Characterization of Antibodies from Phage Display Libraries against Internalizing Membrane Antigens Johan Fransson and Carl A. K. Borrebaeck Artificial Membrane Models for the Study of Macromolecular Delivery Lena Mler and Astrid Grslund Enhanced Delivery of Macromolecules into Cells by Electroendocytosis Alexander Barbul, Yulia Antov, Yosef Rosenberg and Rafi Korenstein In Vitro Systems for Studying Epithelial Transport of Macromolecules Nicole Daum, Andrea Neumeyer, BirgitWahl, Michael Bur, Claus-Michael Lehr Preparation of Macromolecule-containing Dry Powders for Pulmonary Delivery Kelly S. Kraft and Marshall Grant Macromolecular Delivery Across the Blood-Brain Barrier Kullervo Hynynen Positron Emission Tomography (PET) and Macromolecular Delivery In Vivo Ludwig G. Strauss and Antonia Dimitrakopoulou-Strauss
