Description
1. Protein arginylation: milestones of discovery Anna Kashina 2. Recollection of how we came across the protein modification with amino acids by aminoacyl tRNA-protein transferase Hideko Kaji and Akira Kaji 3. Arginyltransferase: a personal and historical perspective Richard L. Soffer 4. Arginylation in a Partially Purified Fraction of 150,000xg Supernatants of Axoplasm and Injured Vertebrate Nerves Nicholas A. Ingoglia 5. Preparation of ATE1 enzyme from native mammalian tissues Anna Kashina 6. Correlated measurement of endogenous ATE1 activity on native acceptor proteins in tissues and cultured cells to detect cellular aging Hideko Kaji and Akira Kaji 7. Assaying the Post-translational Arginylation of Proteins in Cultured Cells Mauricio R. Galiano and Marta E. Hallak 8. Assaying ATE1 activity in yeast by -gal degradation Anna Kashina. 9. Assaying arginylation activity in cell lysates using a fluorescent reporter Akhilesh Kumar and Fangliang Zhang. 10. Assaying intracellular arginylation activity using a fluorescent reporter. Brittany MacTaggart and Anna Kashina 11. Bacterial expression and purification of recombinant arginyltransferase (ATE1) and Arg-tRNA synthetase (RRS) for arginylation assays Junling Wang and Anna Kashina 12. Preparation of tRNAArg for arginylation assay by in vitro transcription by Irem Avcilar-Kucukgoze and Anna Kashina 13. Preparation of an enriched tRNAArg fraction for arginylation by expression in E. coli Irem Avcilar-Kucukgoze, Howard Gamper, Jr., Ya-Ming Hou, and Anna Kashina 14. Enzymatic aminoacylation of tRNAArg using recombinant Arg-tRNA synthetase Irem Avcilar-Kucukgoze and Anna Kashina 15. Assaying ATE1 activity in vitro Junling Wang and Anna Kashina. 16. High throughput arginylation assay in microplate format Sougata Saha, Junling Wang, and Anna Kashina 17. Assaying for arginyltransferase activity and specificity by peptide arrays Junling Wang and Anna Kashina. 18. Assay of arginyltransferase activity by a fluorescent HPLC method Koichi Takao 19. Identification of arginylated proteins by mass spectrometry Anna Kashina and John R. Yates, III 20. Analysis of arginylated peptides by subtractive Edman degradation. Anna Kashina and John R. Yates, III 21. Transferase-Mediated Labeling of Protein N-Termini with Click Chemistry Handles Anne M. Wagner, John B. Warner, Haviva E. Garrett, Christopher R. Walters, and E. James Petersson. 22. Synthesis of peptides and proteins with site-specific glutamate arginylation Marie Shimogawa, Yun Huang, Buyan Pan, and E. James Petersson. 23. Reconstitution of the arginyltransferase (ATE1) iron-sulfur cluster Verna Van and Aaron T. Smith. 24. N-terminal arginylation pulldown analysis using the R-catcher tool Taewook Seo, Goeun Han, and Hyunjoo Cha-Molstad 25. Identification of protein arginylation by encapsulated N-terminal peptide enrichment method Shinyeong Ju and Cheolju Lee. 26. Monitoring the Activation of Selective Autophagy via N-terminal Arginylation. Chang Hoon Ji, Su Bin Kim, Min Ju Lee, and Yong Tae Kwon 27. Analyzing the Interaction of Arginylated Proteins and Nt-Arg-Mimicking Chemical Compounds to N-Recognins Chang Hoon Ji, Ah Jung Heo, and Yong Tae Kwon 28 Synthesis of Stably Charged Arg-tRNAArg for Structural Analysis Yuka Yamaki, Howard Gamper, and Ya-Ming Hou 29. A High-Throughput Colorimetric Microplate Assay for Determination of Plasma Arginase Activity Natalie J Smith, Mahnaz Maddahfar, Bavani Gunasegaran Helen M McGuire, and Barbara Fazekas de St Groth. 30 Development of new tools for the studies of protein arginylation Anna Kashina
